Recent News from the Gray Lab

Parallel non-ionotropic and ionotropic requirements for NMDA-mediated superoxide production

In this collaboration with the Swanson lab at UCSF, we show that NMDA-mediated superoxide production requires both a source of calcium influx and agonist binding to the glutamate site of the NMDA receptor (GluN2B subunit to be specific). Thus, both Ca2+ and agonist -binding are necessary, but neither is sufficient.  This is the first report demonstrating this dual requirement and I predict this will be rule rather than the exception for NMDA receptor function.

https://www.nature.com/articles/s41598-018-35725-5

 

Intracellular MK801 does not fully block NMDA receptors

MK801 is an open-channel blocker of the NMDA receptor pore. That is, when the receptor is activated and the channel opens, MK801 enters the pore and blocks it. Usually, MK801 is used extracellularly, but multiple studies have included it in the intracellular pipette solution during whole-cell patch clamp recordings. We show here that MK801 has a much lower effective affinity for NMDA receptors when used intracellularly, suggesting caution is needed when interpreting such studies.